BioSpec-nano Micro-volume Spectrophotometer from Shimadzu

Product Specs
ItemBioSpec-nano Micro-volume Spectrophotometer
CompanyShimadzu
Price Get Pricing Get Quote for this Product
Catalog NumberBioSpec-nano
Accuracy1 nm
QuantityOne Spectrophotometer
Sample Volume1 to 2µL
Light SourceXenon flash lamp
Wavelength(s)220 to 800 nm
Spectral bandwidth3 nm
Wavelength Accuracy± 1 nm
DetectorPhoto diode array
TypeUV-Vis
ApplicationsDNA, RNA, Protein analysis / Photometric measurement
Sample Type1 to 2 µL samples

Specifications/Features

Automated measurements

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Shimadzu

7102 Riverwood Drive

Columbia, Maryland

United States

Phone: 1-800-477-1227
1-800-477-1227
1-410-381-1222

Company Profile

Website: https://www.ssi.shimadzu.com/

(1) Effect of light conditions on trophic level and gene expression of partially mycoheterotrophic orchid, Cymbidium goeringiiPlant Signaling & BehaviorDecember 31, 2023Keita Chagi, Hiroaki Komoda, Masashi Murakamielectrophoresis on agarose gel was performed to check the RNA degradation. RNA purity was estimated using a BioSpec-nano (Shimadzu, Japan). The cDNA library was constructed using TruSeq RNA Sample Prep Kits. Paired-end (150

(2) Mitochondrial DNA copy number in patients with systemic sclerosisFront Mol BiosciDecember 14, 2023Anastasia I. Bogatyreva, Elena V. Gerasimova, Tatiana V. Kirichenko, Yuliya V. Markina, Taisiya V. Tolstik, Diana G. Kiseleva, Tatiana V. Popkova, Alexander M. Markinthe manufacturer’s protocol. The concentration and purity of the obtained DNA were determined using a BIOSPEC-NANO spectrophotometer (Shimadzu, Japan). 2.4 Quantitative determination of mtDNA copies The copy number

(3) Cloning and Characterization of Yak DHODH Gene and Its Functional Studies in a Bisphenol S-Induced Ferroptosis Model of Fetal FibroblastsAnimals (Basel)December 13, 2023Hongmei Xu, Yueyue Li, Qiao Li, Zifeng Ma, Shi Yin, Honghong He, Yan Xiong, Xianrong Xiong, Daoliang Lan, Jian Li, et al.Anhui, China). The concentration of RNA was determined using a UV-visible spectrophotometer (Shimadzu, Biospec-nano, Shimadzu, Japan) at the wavelength of 260 nm. Afterwards, 1 μg of total RNA was reverse-transcribed

(4) Polyhexamethylene biguanide promotes adaptive cross-resistance to gentamicin in Escherichia coli biofilmsFront Cell Infect MicrobiolDecember 11, 2023Raphaël Charron, Pierre Lemée, Antoine Huguet, Ornella Minlong, Marine Boulanger, Paméla Houée, Christophe Soumet, Romain Briandet, Arnaud Bridierinstructions (Macherey-Nagel, Düren, Germany). The concentration and quality of DNA were checked using a BioSpec-nano spectrophotometer (Shimadzu, Marne la Vallée, France). Illumina sequencing was performed on the strains

(5) Microsatellites reveal divergence in population genetic diversity, and structure of osyris lanceolata (santalaceae) in Uganda and KenyaBMC Ecol EvolDecember 8, 2023Ben Belden Mugula, S. F. Omondi, Manuel Curto, Samuel Kuria Kiboi, James Ireri Kanya, Anthony Egeru, Paul Okullo, Harald Meimberg(acetyl-trimethyl-ammonium-bromide) protocol [34]. The DNA samples were quantified using a nanodrop spectrophotometer (BioSpec-nano, Shimadzu Biotech, Hamburg, Germany) to check the concentration and purity. After quantification, the

(6) Chromosome-Scale Genome Assembly of the Freshwater Snail Semisulcospira habei from the Lake Biwa Drainage SystemGenome Biology and EvolutionNovember 28, 2023Osamu Miura, Atsushi Toyoda, Tetsuya Sakuraidissolved in Tris-HCl buffer. The purity and concentration of the extracted HMW-gDNA were evaluated using BioSpec-nano spectrometer (Shimadzu, Japan) and Qubit fluorometer (Thermo Fisher Scientific, United States). The

(7) Rat and mouse cardiomyocytes show subtle differences in creatine kinase expression and compartmentalizationPLOS ONENovember 27, 2023Jelena Branovets, Kärol Soodla, Marko Vendelin, Rikke Birkedalweight and CS activity. The protein content in cardiomyocytes was measured spectrophotometrically in a BioSpec-nano (Shimadzu Scientific Instruments Inc., Columbia, MD) as previously described [21]. Statistics The

(8) Fecal prevalence of Campylobacter spp. in house dogs in Lebanon: A pilot studyVeterinary WorldNovember 11, 2023Ghassan Ghssein, Rana Barakat, Nada Nehme, Rana Awada, Hussein F. Hassanwas stored at −20°C and used for PCR within a week, following DNA quantification using a nanodrop (BioSpec-nano Micro-Volume Spectrophotometer by Shimadzu, Columbia, Maryland, USA). Campylobacter detection using PCR method Polymerase chain reaction

(9) Prognostic Potential of Galectin-9 mRNA Expression in Chronic Lymphocytic LeukemiaCancers (Basel)November 11, 2023Agnieszka Bojarska-Junak, Wioleta Kowalska, Sylwia Chocholska, Agata Szymańska, Waldemar Tomczak, Michał Konrad Zarobkiewicz, Jacek Rolińskiextracted from 5 × 106 PBMCs by QIAamp DNA Blood Mini Kit (Cat No. 51104, Qiagen, Hilden, Germany). The BioSpec-nano spectrophotometer (Shimadzu, Kyoto, Japan) was utilized to estimate the concentration and purity of

(10) Tomato root-associated Sphingobium harbors genes for catabolizing toxic steroidal glycoalkaloidsmBioOctober 31, 2023Masaru Nakayasu, Kyoko Takamatsu, Keiko Kanai, Sachiko Masuda, Shinichi Yamazaki, Yuichi Aoki, Arisa Shibata, Wataru Suda, Ken Shirasu, Kazufumi Yazaki, et al.precipitation method and dissolved in 100 µL of TE buffer. The DNA concentration was measured using a BioSpec-nano instrument (Shimadzu, Kyoto, Japan) and a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham,

(11) Complete genome sequences of α-glucosidase-positive and -negative strains of Nocardia seriolae from Seriola species in JapanMicrobiology Resource AnnouncementsOctober 19, 2023Kousuke Umeda, Yuta Matsuura, Yoshiko Shimahara, Tomokazu Takano, Tomomasa MatsuyamaMaxwell 16 Tissue DNA Purification Kit (Promega Corporation, Madison, WI, USA). DNA concentration and quality were assessed using a BioSpec-nano (Shimadzu Corporation, Kyoto, Japan). All the kits used for DNA extraction and library preparation were

(12) Persistent dyslipidemia increases the longitudinal changes in telomere lengthLipids in Health and DiseaseOctober 18, 2023Xiaowei Liu, Tao Ma, Chan Yang, Juan Li, Yuhong Zhang, Yi Zhaoleukocytes, D3392-04 DNA Blood Mid Kit (Bao Bioengineering Co., Ltd., Japan) was utilized in this study. The Biospec-nano instrument (Shimadzu, Japan) was used to measure DNA content and quality. Then, OD260/OD280 ratio was

(13) Multi-Organ Nutrigenomic Effects of Dietary Grapes in a Mouse ModelAntioxidants (Basel)October 1, 2023Asim Dave, Eun-Jung Park, John M. Pezzutoquantity and quality of the extracted RNA samples, spectrophotometric measurements were taken using BioSpec-nano (Shimadzu, Tokyo, Japan), and the integrity of the RNA samples was evaluated via QIAxcel® capillary electrophoresis

(14) A clash on the Toll pathway: competitive action between pesticides and zymosan A on components of innate immunity in Apis melliferaFrontiers in ImmunologySeptember 11, 2023Dani Sukkar, Ali Kanso, Philippe Laval-Gilly, Jairo Falla-Angelsample was suspended in 40 µl in RNase-free water. Sample concentration and purity were quantified by BioSpecNano spectrophotometer (Shimadzu corps™). 2.4 cDNA synthesis RNA of each sample was reverse transcribed

(15) Association between Combination Antiretroviral Therapy and Telomere Length in People Living with Human Immunodeficiency VirusBiology (Basel)September 5, 2023Ena Bukic, Jelena Milasin, Bosko Toljic, Jelena Jadzic, Djordje Jevtovic, Bozana Obradovic, Gordana Dragovicboth the concentration and quality of isolated DNA were evaluated by the spectrophotometric method (BioSpec-nano, Shimadzu Biotech, Kyoto, Japan). Only samples with sufficient DNA concentration and satisfactory purity

(16) Single-cell multi-omics identifies chronic inflammation as a driver of TP53-mutant leukemic evolutionNature GeneticsSeptember 4, 2023Alba Rodriguez-Meira, Ruggiero Norfo, Sean Wen, Agathe L. Chédeville, Haseeb Rahman, Jennifer O’Sullivan, Guanlin Wang, Eleni Louka, Warren W. Kretzschmar, Aimee Paterson, et al.Fisher Scientific) according to the manufacturer’s recommendations. DNA amplification was checked using BioSpec-nano spectrophotometer (Shimadzu) with expected concentrations between 2,500 ng μl−1 and 3,400 ng μl−1. DNA

(17) Microbial communities inhabiting shallow hydrothermal vents as sentinels of acidification processesFrontiers in MicrobiologySeptember 1, 2023Erika Arcadi, Carmen Rizzo, Rosario Calogero, Valentina Sciutteri, Francesco Fabiano, Pierpaolo Consoli, Franco Andaloro, Teresa Romeoextracts obtained from filters and sediments was determined by spectrophotometric measurements with a BioSpec-Nano spectrophotometer (Shimadzu Corporation). DNA samples were stored at −20°C until further analyses. Bacterial

(18) Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of Aristolochia manshuriensisJournal of Functional BiomaterialsSeptember 1, 2023Yulia A. Yugay, Maria R. Sorokina, Valeria P. Grigorchuk, Tatiana V. Rusapetova, Vladimir E. Silant’ev, Anna E. Egorova, Peter A. Adedibu, Olesya D. Kudinova, Elena A. Vasyutkina, Vladimir V. Ivanov, et al.water. 2.2. AgNPs Characterization The UV-Vis spectral analysis of AgNPs was carried out using a BioSpec-nano spectrophotometer (Shimadzu, Kyoto, Japan), with a resolution of 1 nm and a pathlength of 0.2 mm. To

(19) Exploring novel genetic and hematological predictors of response to neoadjuvant chemoradiotherapy in locally advanced rectal cancerFrontiers in GeneticsAugust 31, 2023Mladen Marinkovic, Suzana Stojanovic-Rundic, Aleksandra Stanojevic, Marija Ostojic, Dusica Gavrilovic, Radmila Jankovic, Natasa Maksimovic, Rafael Stroggilos, Jerome Zoidakis, Sergi Castellví-Bel, et al.Manchester, United Kingdom). RNA quality and concentration were determined spectrophotometrically using BioSpec-nano (Shimadzu Scientific Instruments, Kyoto. Japan). The complementary DNA (cDNA) was accessed from 1 µg

(20) ZNF671 methylation test in cervical scrapings for cervical intraepithelial neoplasia grade 3 and cervical cancer detectionCell Rep MedAugust 15, 2023Peng Zhu, Jing Xiong, Ding Yuan, Xiang Li, Lili Luo, Ju Huang, Binbin Wang, Quanfang Nie, Shuli Wang, Liying Dang, et al.(Magen Biotech, Guangzhou, China). The concentrations of gDNA in each sample were measured using a BioSpec-nano spectrophotometer (Shimadzu Corporation, Tokyo, Japan). HrHPV DNA test DNA were prepared as described

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